Jeg-3 cell line

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August undefined, 2024

WebJEG, JEG-3 Human choriocarcinoma One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the …

JEG-3 placental cells in toxicology studies: a promising tool ... - PubMed

Web31 mar 2024 · JEG-3 cells prepared in our incubation conditions were renamed JEG-Tox cells. As placental alterations are mainly triggered by uncontrolled apoptosis, we second … Web15 mar 2024 · After electroporation and drug selection, a successful HLA-G knockout JEG-3 cell line (JEG-3 KO cells) was established, and its efficacy was first validated by PCR and western blotting . Additional functional validation was performed on the well-established functions of HLA-G, including the inhibition of natural killer cell cytotoxicity and the … government stimulus package for farmers

The choriocarcinoma cell line JEG-3 upregulates regulatory T cell ...

WebNorfluoxetine increased MMP-9 activity in JEG-3 cells by 2.0% at 0.03 μM and by 43.9% at 3 μM, but decreased MMP-9 activity in HIPEC cells by 63.7% at 3 μM. Sertraline at 0.03 μM increased mRNA level of TIMP-1 in JEG-3 cells by 36% and that of ADAM-10 by 85% and 115% at 0.3 and 3 μM, respectively. Web1 set 2024 · Co-culture of human decidual or peripheral NK cells with a trophoblast-like cell line JEG-3 infected with L. monocytogenes significantly reduced intracellular bacteria levels but did not result in ... Web31 mar 2024 · The choriocarcinoma-derived JEG-3 cell-line (ATCC HTB-36, Manassas, VA, USA), was grown as recommended by ATCC: ... JEG-3 cells in 2.5% FBS released similar levels of hCG, hPL, and estradiol to JEG-3 cells in 10% FBS, and thus maintain the endocrine function of human placenta. childrens nutritionist

JEG-3 cell line AcceGen

WebIt was postulated that 3D cell culture models more accurately reflect the complex tissue physiology and morphology in comparison to 2D cell monolayers. Currently, there is a shortage of well-characterized and easily maintainable high-throughput experimental models of the human placenta. Here, we characterized three different 3D cultures (e.g., … WebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. ... JEG-3 Product Name: JEG-3. Price: Get Quote: Cat.No: ABC-TC0467. Species: Human. Size/Quantity: 1 vial. Biosafety Level: 1. ... Cell line releases chorionic gonadotrophin and somatomammotrophin and ... childrens oakland dermatologyWebPeripheral blood mononuclear cells and placental tissue were collected. The human JEG3 trophoblast cell line was cultivated in 5.5 mmol/L or 30 mmol/L glucose and transfected with wild-type(wt)-p66 Shc and p66 Shc si RNA. P66 Shc and Drp1 m RNA levels were detected by quantitative real-time polymerase chain reaction. government stimulus package application

"WebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. ... JEG-3 Product Name: … " - Jeg-3 cell line

Jeg-3 cell line

Application of a JEG-3 organoid model to study HLA-G function in …

WebOn day 0, JEG-3 cultures received resting or stimulated PBMCs in a 1:25 or 1:50 ratio of JEG-3 cells to PBMCs. This ratio was calculated based on the initial number of JEG-3 seeded in the culture plates. A low dose of IL-2 (Sigma) was added at this time and again on day 3 to promote PBMC survival. Treated JEG-3 cell co-cultures were performed ... Web15 gen 2024 · JEG-3 cells synthesize placenta-specific hormones, such as human chorionic gonadotrophin [1] and, in contrast to most other “trophoblastic” cell lines, …

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Web13 apr 2024 · To verify the function of miR-526b-5p on the trophoblast cells and explore the intrinsic molecular mechanism, transcriptome sequencing was performed on the HTR8 … WebMuys BR, Lorenzi JC, Zanette DL, et al. Placenta-enriched LincRNAs MIR503HG and LINC00629 decrease migration and invasion potential of JEG-3 cell line. PLoS One. 2016;11(3):e0151560. 10. Mogilyansky E, Rigoutsos I. The miR-17/92 cluster: a comprehensive update on its genomics, genetics, functions and increasingly important …

WebThe cytotoxicity of eight perfluorinated chemicals (PFCs), namely, perfluorobutanoic acid (PFBA), perfluorohexanoic acid (PFHxA), perfluorooctanoic acid (PFOA), perfluorononanoic acid (PFNA), perfluorododecanoic acid (PFDoA), perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHxS) and perfluorooctanesulfonate (PFOS) was assessed … Web30 ott 2024 · The most commonly used cell line in studies on human placental transport is the choriocarcinoma cell line BeWo, ... The effects of aflatoxin B1 on transporters and steroid metabolizing enzymes in JEG-3 cells. Toxicol. Lett. 2013, 218, 200–206. [Google Scholar] Chen, Z.S ...

Web6 ott 2005 · Like the native trophoblast HLA-G, the JEG-3 cell HLA-G heavy chain is recognized by both of the monoclonal antibodies (mAb) (2C/C8 and 3C/G4) used in our enzyme-linked immunosorbent assay (ELISA) (see below). Using a cell line had the advantage of avoiding contamination by other cell types that may also have … WebFigure 10 The effect of overexpression or silencing of hsa-miR-29b-3p, hsa-miR-519c-3p and hsa-miR-520a-5p on cell proliferation in JEG-3 and JAR cells. Viability of JEG-3 and JAR cells transfected with hub DEM mimics or inhibitors measured by CCK-8 (A–D) and EdU assays (E and F). The percentage of Edu positive JEG-3 and JAR.

WebOne of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic …

Web31 mar 2024 · JEG-3 placental cells in toxicology studies: a promising tool to reveal pregnancy disorders Anat Cell Biol. 2024 Mar 31;54 (1):83-92. doi: 10.5115/acb.20.234. Authors Elodie Olivier 1 , Anaïs Wakx 1 , Sophie Fouyet 1 , Mélody Dutot 1 2 , … government stock trackerWeb19 lug 2024 · Here, we present a comparative study on the functional aspects of the choriocarcinoma cell lines BeWo, JAR and Jeg-3, as well as the first trimester … childrens nutrition shakesWeb9 mag 2024 · The miR-371-3 cluster (including miR-371, -372, and -373) is expressed in primary trophoblast cells, JEG-3 cells, and their hybrids, but not in immortalized HTR-8/SVneo Mice have homologous ... regulation of Wnt/ β-catenin signaling pathway by miR-424-5p and affects invasion and migration of the trophoblastic cell line HTR-8/SVneo childrens oakland ophthalmologyWeb12 nov 2024 · The JEG-3 cell line was transfected with pSpCas9 vector cloned with each sgRNA and analysed as follows. First, HLA-G expression was measured by WB and FC on JEG-3 edited cells. childrens obituary templateWeb10 nov 2006 · The JEG-3 cell line are human choriocarcinoma cells derived from malignant placental tissues. As in placenta, JEG-3 cells endogenously express aromatase activity … childrens o and pWeb13 nov 2013 · Line Spacing: Column ... MCF7 and HepG2 cells were transiently transfected with 3 μg HIS-FLAG-tagged WT Nr5a1 or Nr5a1 in which lysine 194 (K194R), glutamic acid 199 (E199A), or both lysine 194 and glutamic acid 199 (K194RE199A) were mutated to arginine or alanine. government stock for ltcWebCell line: JEG-3 DSMZ no.: ACC 463 Species validation: human ( Homo sapiens) Cell type: choriocarcinoma Origin: childrens oakland hospital in the usa