Hif1α antibody
WebAnti-HIF-1 alpha antibody [EPR16897] (ab179483) Research with confidence – consistent and reproducible results with every batch Long-term and scalable supply – powered by … Web9 de jun. de 2024 · Chromatin was diluted and input chromatin was collected. Remaining chromatin was used for ChIP by adding 4 μg mouse anti–HIF-1α (Abcam; ab1) as the antibody of interest, 4 μg mouse anti-histone H3 (Abcam, ab1220) as a positive control, and mouse anti-GFP antibody (Abcam, ab1218) as a negative control. Antibodies were …
Hif1α antibody
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WebHIF-1α Antibody detects endogenous levels of total HIF-1α protein. The antibody does not cross-react with other family members at physiological conditions, and does not detect significant levels of hydroxylated HIF-1α. … WebTranswell chamber was purchased from Corning (NY, USA). The antibodies to HIF-1α ( GTX127309) were purchased from GeneTex (TX, USA). The antibodies to Phospho- JNK1/2 /3 ( AP0631) and JNK1 / JNK3 ( A5051) (3) Neuroinvasion of SARS-CoV-2 in human and mouse brain bioRxiv September 27, 2024 Eric Song et al.
WebAntibodies that detect HIF1A can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. … Web11 de abr. de 2024 · HIF-1α activates TMEM237 transcription in hypoxic HCC cells. We previously investigated hypoxia-induced transcripts in Hep3B cells using a microarray assay [].The microarray data (GSE155505) showed that TMEM237 was significantly upregulated in Hep3B cells under hypoxic conditions (Fig. 1a). Then, RT‒qPCR and Western blotting …
WebView Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ChIP, Flow, ICC/IF, IHC + more. Species Reactivity: Human, Mouse, Rat + … WebKnockout Validated Rabbit Polyclonal anti-HIF-1 alpha Antibody - BSA Free. Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, Dual ISH-IHC + more. Reactivity: …
WebHIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer …
WebDetection of Human HIF‑1 alpha /HIF1A by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 150 µM CoCl 2 for 16 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat HIF-1a/HIF1A Antigen-affinity Purified Polyclonal Antibody, followed by HRP-conjugated Anti … how much should a 5 ft 12 year old girl weighWebImmunoprecipitation of HIF-1α from lysate of Hep G2 cells treated with cobalt chloride (100 µM, 4 h). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900, and lane 3 is HIF-1α (D1S7W) XP ® Rabbit mAb. Western blot analysis was performed using HIF-1α (D1S7W) XP ® Rabbit mAb. Anti-rabbit IgG, HRP-linked … how do spiders huntWebHIF-1α Antibody (28b) is an IgG κ mouse monoclonal HIF-1 alpha antibody (also designated HIF1A antibody or HIF-1 alpha antibody) suitable for the detection of the … how do spiders live through the winterWeb18 de mar. de 2024 · Membranes were blocked with 5% milk powder in 1× PBS-T for 1 h and then incubated overnight at 4 °C with a mouse anti-HIF-1α monoclonal antibody in … how do spiders biteWebHIF-1α Antibody (C-19) has been replaced by a more specific monoclonal antibody, HIF-1α (28b) that provides a stronger signal & more reproducible data. Choose a Store Santa … how much should a 5 ft 4 inch woman weighWeb30 de out. de 2024 · HIF-1α Antibody (H-206) is a rabbit polyclonal IgG; 200 µg/ml; epitope corresponding to amino acids 575-780 mapping near the C-terminus of HIF-1α of human origin; Discontinued polyclonal antibody; more . HIF-1α (H-206) has been discontinued and replaced by HIF-1α (28b): sc-13515. QUICK LINKS. how do spiders hibernateWeb6 de mai. de 2013 · GST-p300 CH1 protein (∼10 µg GST only as a negative control) was mixed with His-HIF1α FL, 15 µl of glutathione sepharose 4 Fast Flow beads, and novobiocin (100–400 µM). Bound proteins were then detected by Western blotting with anti-HIF1α antibody. Quantified HIF1α is depicted in the graph (lower panel). how much should a 5 foot child weigh