Fitc-conjugated pac-1
WebNov 19, 2014 · FITC-RGD2, FITC-3P-RGD2, and FITC-Galacto-RGD2 were prepared, and their integrin αvβ3/αvβ5 binding affinity was determined using the displacement assay against (125)I-echistatin bound to U87MG glioma cells. IC50 values of FITC-Galacto-RGD2, FITC-3P-RGD2, and FITC-RGD2 were calculated to be 28 ± 8, 32 ± 7, and 89 ± 17 nM, … WebApr 4, 2024 · PAC-1 Antibody (4O21) is available as both the non-conjugated anti-PAC-1 antibody form, as well as multiple conjugated forms of anti-PAC-1 antibody, including agarose, HRP, PE, FITC and …
Fitc-conjugated pac-1
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WebThe FITC-conjugated antibodies employed in a laboratory for the DFA test may be developed in house or commercially supplied. Some laboratories use a cocktail of monoclonal antibodies raised in mice against prominent antigens (e.g., nucleoprotein or glycoprotein), while others use a polyclonal antirabies antiserum raised in suitable … WebThe PAC-1 antibody is derived from the fusion of SP-2 mouse myeloma cells with splenic lymphocytes from BALB/c mice that were immunized with human platelets. The PAC-1 recognizes an epitope on the glycoprotein IIb/IIIa (gpIIb/IIIa, α IIb β 3) complex of activated platelets at or near the platelet fibrinogen receptor.
WebIn Fig. 1a, we used FITC- conjugated anti-human fibrinogen antibody to highlight intercellular reactivity (white dots, red arrow). The round structures (blue arrow) seem to be a series of TTS ... WebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on …
WebClone HIT3a. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD3 (Cat. No. 561802/555539; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. … WebJun 1, 2002 · On human platelets, the high affinity conformation of alphaIIbbeta3 is detected by the monoclonal antibody (mAb), PAC-1. However, a reagent with binding specificity to high affinity mouse alphaIIbbeta3 has not been described so far. ... JON/A was conjugated with fluorescein isothiocyanate (JON/A(FITC)) or with R-phycoerythrin (JON/A(PE)) and ...
WebFITC conjugates of antibodies, lectins, hormones, and growth factors have been used in a variety of immunohistochemical and flow cytometry applications. The protocols have been optimized for antibodies, but may be adapted to other proteins by the end user.
Webphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and fluorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). cubehamster\u0027s fully automatic mining drillWebConjugation: FITC: Tested applications: FACS, ICC, IF, IP: Immunogen: Human platelets: Target: PAC-1 (FITC). The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation. cubehandlecapWebSep 18, 2024 · FITC-conjugated anti-human CD62P (P-selectin) and FITC-conjugated anti-human PAC-1 were purchased from BioLegend (California, USA). Fluo-3 AM calcium indicators and lactate dehydrogenase (LDH) … east cleveland contractor registrationWebAug 1, 2003 · MFI of PAC-1-FITC was assessed in 5 whole blood samples immediately, 15, 30, and 60 min after antibody labelling with PAC-1 and CD42b MoAbs. cube handschuhe langfingerWebThe PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a IIb ß 3. PAC-1 binding to fibrinogen is pH and Ca 2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher. cube halliwell road boltonWeb2. Slowly add FITC to protein with stirring. Cover with foil and stir 2 hours at room temperature. 3. Separate conjugate from free FITC on G-25 column. Collect fractions. 4. Pool fractions containing conjugate. 5. Determine F/P ratio of conjugate spectrophotometrically. 6. Stabilize with 1% bovine serum albumin and 0.1% sodium … east cleveland cemetery cleveland ohioWeball FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1" solutions. 5. Prepare the required dilution of FITC in 0.1 M carbonate-bicarbonate buffer as directed in ... cubehamster minecraft