Csfv shimen strain

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WebMar 13, 2024 · As shown in Fig. 4A,B, our method could detect the CSFV Shimen strain or the HCLV strain, while negative results were obtained … WebMar 8, 2024 · The Shimen strain of classical swine fever (CSF) virus (CSFV) causes CSF, which is mainly characterised by disseminated intravascular haemorrhage. Macrophages …

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WebDec 1, 2013 · The strains of CSFV (Shimen and C) used were obtained from the Control Institute of Veterinary Bioproducts and Pharmaceuticals (China). Virus strains were … WebClassical swine fever (CSF) caused by the classical swine fever virus (CSFV) has resulted in severe losses to the pig industry worldwide. It has been proposed that lipid synthesis is essential for viral replication, and lipids are involved in viral protein maturation and envelope production. However, the specific crosstalk between CSFV and host cell lipid metabolism … songs with cow in the title

Efficient and stable rescue of classical swine fever virus ... - Springer

WebShimen strain is highly virulent while HCLV, also referred to as C-strain, is a live attenuated vaccine strain considered to be one of the most effective and safest live … WebJan 2, 2024 · The CMV promoter launched CSFV Shimen strain infectious cDNA was assembled in a bacterial artificial chromosome. • The progeny viruses were rescued efficiently and was identical to the wild type parent virus. • A recombinant virus CSFV-mCherry expressing the mCherry reporter gene was established. • WebMay 1, 2024 · The chimeric CSFV/BVDV fragment consisted of the E2 gene of BVDV and p7 gene and incomplete NS2–3 coding region of CSFV, which was obtained from overlap PCR amplification using the cDNAs of BVDV Hubei strain and CSFV Shimen strain as templates. The pCSFV/bE2 mutants were constructed using genetic approaches based … small glass angel figurines

Generation and Efficacy Evaluation of Recombinant …

Genetic characterization of E2 gene of classical swine fever

WebAug 17, 2024 · In this study, we demonstrated that P108 and T109 on the E2 glycoprotein together with the E rns glycoprotein of the rabbit-adaptive C-strain confer adaptation to rabbits on the highly virulent CSFV Shimen strain by affecting viral entry during infection but do not attenuate the Shimen strain in pigs. Our results provide vital information on ... WebJul 1, 2015 · The Shimen strain infection resulted in a significant activation of IFN regulatory factor IRF7 and suppression of IRF3. These data provided clues for … songs with cross in the titleWebSep 8, 2014 · The virulent Shimen strain of CSFV, which is the standard virulent strain that has been used for vaccine potency tests in China since the 1950s, was used in this … songs with cover versions

"WebNov 27, 2015 · The highly virulent CSFV Shimen strain (wt-Shimen) was used as a wild-type control in the experiment. EGFP-CSFV is an EGFP-tagged CSFV derived from pEGFP-CSFV [ 10 ]. vSM-HCLV3′UTR is a chimeric CSFV based on the wt-Shimen backbone with the 3′-UTR replacement by that of HCLV [ 8 ], which was generated in a way similar to … " - Csfv shimen strain

Csfv shimen strain

Generation and evaluation of a chimeric classical swine fever virus ...

WebSep 13, 2013 · CSFV Shimen strain was used as challenge virus in this study and PAMs were cultured in vitro. Interferon regulatory factor (IRF)-3 and nuclear factor-kappa B (NF-κB) translocation was detected using immunofluorescent staining; RIG-I, MDA5, interferon promoter-stimulating factor 1 (IPS-1), IRF-3 and NF-κB expression was measured by … WebDocument: In a combined vaccine approach, SFV DNA vectors and recombinant adenovirus expressing the classical swine fever virus (CSFV) E2 glycoprotein elicited higher titers of neutralizing antibodies in pigs [26] . After challenges with the virulent CSFV Shimen strain, no symptoms of viremia were observed, for the combined vaccine, whereas vaccination …

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WebMar 13, 2024 · As shown in Fig. 4A,B, our method could detect the CSFV Shimen strain or the HCLV strain, while negative results were obtained with other virus strains. Three independent reproducibility tests ... WebNov 29, 2012 · In the present study, CSFV Shimen strain full-length genomic cDNA carrying a genetic tag (AvaI site) was cloned downstream of the cytomegalovirus (CMV) …

WebApr 2, 2024 · A multiplex reverse transcription polymerase chain rection (mRT-PCR) was developed for simultaneous detection of four RNA viruses in swine. The conserved target sequences directed to classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV) and … WebJan 3, 2024 · The highly pathogenic CSFV Shimen strain does not infect rabbits irrespective of high genomic similarity with C-strain (97.3% similarity at amino acid …

WebA Biblioteca Virtual em Saúde é uma colecao de fontes de informacao científica e técnica em saúde organizada e armazenada em formato eletrônico nos países da Região Latino-Americana e do Caribe, acessíveis de forma universal na Internet de modo compatível com as bases internacionais. WebJul 21, 2024 · All the vaccinated piglets were challenged intramuscularly with 10 5 TCID 50 CSFV Shimen strain at 35 dpi. For single dose immunization schedule, twelve 4-week-old pigs were randomly divided into three groups (A, B and C, n = 4), and subject to an immunization and challenge trial. In brief, Groups A and B were respectively immunized …

WebJun 25, 2024 · The CSFV Shimen strain, a highly virulent strain isolated in China in 1945, were also used as a reference challenge virus in the present study. Primer design and …

WebMar 1, 2024 · The CSFV Shimen strain is a highly virulent strain isolated in China in 1945, which has been used as a reference challenge virus to evaluate the efficacy of CSF vaccines in China. The HLJZZ2014 strain is a subgenotype 2.1d CSFV isolated in 2014 in China (Luo et al., 2016). The CSFV Shimen and HLJZZ2014 strains were propagated … songs with countable and uncountable nounsWebThe CSFV Shimen strain (GenBank accession number AF092248.2) was generated from a cDNA clone (pSPT I / SM) [18] and propagated in PK15 cells. The BVDV I Hubei strain (GenBank accession number ... songs with crazy beatsWebMay 31, 2024 · A full-length cDNA clone of attenuated strain HuN4-F112, obtained by serial cell passage from HP-PRRSV HuN4-F5 (GenBank accession no. EF635006), was used as the backbone for insertion of the E2 gene from the CSFV Shimen strain (GenBank accession no. AF092448) [24]. The resultant recombinant virus was compared to the … small glass and silver coffee tableWebFeb 4, 2024 · Methods Viruses and cells. CSFV strains Shimen (GenBank: FJ598612.1) and HZ08 (GenBank: EF683627) were received from CATG lab,... Construction of … small glass and wood coffee tableWebNov 19, 2024 · Then, the cells were stimulated with ultraviolet-light-inactivated CSFV (Shimen strain; MOI = 1) l. After 24 h of incubation at 37 °C, the cell-free supernatant was removed and detected using a commercially available porcine IL-4 and IFN-γ ELISA kit according to the manufacturer’s protocol. OD values were measured at 450 nm using a ... songs with crime in the titleWeb猪瘟病毒野毒株E2蛋白特异性单抗免疫抑制制备法的建立.pdf small glass angel ornamentsWebDuplex PCR for distinguishing shimen and C-strain of CSFV A duplex PCR for distinguishing the Shimen strain from the C-strain was performed with a total volume of … small glass animal ornaments